Eva receives high profile guests from politics, entertainment, sports and many more every weekday. Together with her guests, she will discuss the news from the past days and to what we may expect this upcoming week

Jinek - Netflix

Type: Talk Show

Languages: Dutch

Status: Running

Runtime: 60 minutes

Premier: 2013-09-08

Jinek - Trans-activating crRNA - Netflix

In molecular biology, trans-activating crRNA (tracrRNA) is a small trans-encoded RNA. It was first discovered in the human pathogen Streptococcus pyogenes. In bacteria and archaea; CRISPR/Cas (clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins) constitute an RNA-mediated defense system which protects against viruses and plasmids. This defensive pathway has three steps. First a copy of the invading nucleic acid is integrated into the CRISPR locus. Next, CRISPR RNAs (crRNAs) are transcribed from this CRISPR locus. The crRNAs are then incorporated into effector complexes, where the crRNA guides the complex to the invading nucleic acid and the Cas proteins degrade this nucleic acid. There are several pathways of CRISPR activation, one of which requires a tracrRNA which plays a role in the maturation of crRNA. TracrRNA is partially complementary to and base pairs with a pre-crRNA forming an RNA duplex. This is cleaved by RNase III, an RNA-specific ribonuclease, to form a crRNA/tracrRNA hybrid. This hybrid acts as a guide for the endonuclease Cas9, which cleaves the invading nucleic acid.

Jinek - See also - Netflix

CRISPR

Jinek - References - Netflix